橙皮素A的NMR研究

橙皮素A是从中药北沙参(Glehnia littoralis Fr. Schmidt ex Miquel)的正丁醇萃 取物中分离得到的一种8-O-4′-型异木脂素苷类化合物. 通过化学方法和波谱分析鉴 定了该 化合物的结构. 采用2D NMR技术对其NMR信号进行了全归属. DMSO-d₆代替CD₃OD作为溶剂，并利用重水交换，能准确读出偶合常数J_7,8值.      

Topological Properties of Urban Public Traffic Networks in Chinese Top-Ten Biggest Cities

We investigate the topological characteristics of complex networks as exemplified by the urban public traffic network （UPTN） in Chinese top-ten biggest cities. It is found that the UPTNs have small world behaviour, by the examination of their topological parameters. The quantitative analysis of the transport efficiency of the UPTNs reveals their higher local efficiency El and lower global efficiency Eg, which coincide well with the status quo of those Chinese cities still at their developing stage. Furthermore, the topological properties of efficiency in the UPTNs are also examined, and the findings indicate that, on the one hand, the UPTNs show robustness to random attacks and frangibility to malicious attacks on a global scale; on the other hand, the interrelation between UPTN efficiency and network motifs deserves our attention. The motifs which interrelate the UPTN efficiency are always triangular-formed patterns, e.g. motifs ID 238, ID 174 and ID 102, etc.     

Taura 综合征病毒主要结构蛋白的表达与纯化

根据Taura综合征病毒(TSV)基因组,设计特异性引物,从感染病毒组织中提取组织总RNA后扩增,分别将3个主要结构蛋白基因VP1、VP2和VP3克隆到pGEM TEasyVector.与表达载体连接后,导入大肠杆菌中诱导表达,并纯化目的蛋白.诱导表达的融合蛋白分子量分别为54.2×103、43×103和57.1×103,在变性条件下过柱纯化VP1和VP2,一次可以纯化10mg以上纯度较高的蛋白.     

Lehmer序列中的平方数

设V_n(R,Q)表示参数为R和Q的Lehmer伴随序列,如果R和Q为互素奇数且D=R-4Q>0,我们找出了满足Q_n(R,Q)或nQ_n(R,Q)是平方数的所有奇数n,这里Q_n(R,Q)R~(1/2)=V_n(R,Q)。     

广义酉矩阵与广义Hermite矩阵

给出了广义酉矩阵与广义(斜)Hermite矩阵的概念，研究了它们的性质及其与酉阵、共轭辛阵、Hermite阵、Hamilton及广义逆矩阵之间的联系；取得了许多新的结果；推广了酉矩阵、Hermite阵与斜Hermite阵间的相应结果，特别将正交阵的广义Cayley分解推广到了广义酉矩阵上；将各类酉矩阵、Hermite矩阵及广义逆矩阵统一了起来．     

无机纳米粒子作为生物探针在生物分析中的研究进展

介绍了无机纳米粒子在生物分析领域的研究进展分别从生物分子与纳米粒子的耦联方式、检测生物分子的纳米金探针、核酸或蛋白质修饰的其它纳米探针以及生物纳米技术的应用前景4个方面对该领域的发展进行了概述。     

铁磁/ 反铁磁双层膜中交换偏置

铁磁/反铁磁交换偏置在巨磁电阻器件中具有重要的应用。引起了物理学及材料学等领域内广大科学家的浓厚兴趣，本文首先阐述了交换偏置的基本性质。然后简述了交换偏置的实验研究方法；最后，着重介绍了几种主要的理论模型。     

丝瓜种子萌芽及子叶生长过程中谷氨酰胺合成酶同工酶的变化

测定了丝瓜种子萌芽及子叶不同生长阶段的谷氨酰胺合成酶(GS)的活性及其同工酶的变化．除了发育上的变化外，光和外源氮对GS活性及其同工酶影响也是显著的．无论外源氮(N)是否存在，GS活性在种子萌芽和子叶生长初期是逐渐升高的，于第6天达到最大，其后活性逐步降低．在光照下，外源氮对GS1诱导作用大于GS2；在无氮下，光对GS2的刺激大于GS1；在暗处，外源氮对GS1和GS2两者都有诱导作用．     

罗氏沼虾幼苗体内一种极小病毒的序列测定与分析

从患罗氏沼虾幼苗肌肉白浊病的幼虾体内分离到两种病毒颗粒,诺达病毒(MrNV)和直径只有15nm的小病毒颗粒(extrasmallvirus,XSV),本文通过建立病毒cDNA文库获得XSV基因组的部分序列,在此基础上合成探针,用从病虾组织中提取的总PNA进行Northern杂交,结果表明XSV基因组至少包括两条RNA片段.利用不同的方法对XSV的两端序列进行克隆和测定,分别得到了872、831和662bp3个相互重叠的序列片段,这3个序列的阅读框编码一个相同的大小在17×103左右的蛋白.     

Synthesis of acetone oxime through acetone ammoximation over TS-1

The ammoximation of acetone to acetone oxime with 30 wt.% hydrogen peroxide over TS-1 has been studied. The conversion of acetone and the selectivity to acetone oxime can reach 99% and 94%, respectively. The by-products are the corresponding hydrazone, imine and small amounts of unidentified compounds. The influence of various parameters such as solvent, temperature, amounts of catalyst and the NH₃ to acetone molar ratio were investigated. This revised version was published online in August 2006 with corrections to the Cover Date.